Journal: Biomaterials Research

Article Title: Enhanced Chondrogenic Differentiation of Electrically Primed Human Mesenchymal Stem Cells for the Regeneration of Osteochondral Defects

doi: 10.34133/bmr.0109

Figure Lengend Snippet: Schematic illustration of electrically primed mesenchymal stem cells (epMSCs) and their chondrogenic potential and regeneration ability in articular cartilage. MSCs, mesenchymal stem cells; ES, electrical stimulation; COL2, type II collagen; SOX9, SRY-box transcription factor 9; ACAN, aggrecan.

Article Snippet: TRIzol reagent and LIVE/DEAD viability/cytotoxicity kit for mammalian cells, mouse COL1 monoclonal antibody (MA1-26771), rabbit type II collagen (COL2) polyclonal antibody (PA1-26206), CD14–phycoerythrin (PE), CD34–PE, CD45–PE, CD73–PE, CD90–PE, CD105–PE, and mouse immunoglobulin G1 kappa isotype control–PE were purchased from Invitrogen (Carlsbad, California, United States).

Techniques:

Journal: Biomaterials Research

Article Title: Enhanced Chondrogenic Differentiation of Electrically Primed Human Mesenchymal Stem Cells for the Regeneration of Osteochondral Defects

doi: 10.34133/bmr.0109

Figure Lengend Snippet: Histological and immunohistochemical (IHC) analyses of cartilage repair in the rat model at weeks 4 and 8 after transplantation. (A) Hematoxylin and eosin (H&E), (B) Masson’s trichrome, (C) Alcian blue, and (D) Safranin-O staining images and (E) type II collagen (COL2) IHC staining images of osteochondral defects at weeks 4 and 8 after transplantation. Scale bar = 500 μm. (F) Quantitative data of cartilage regeneration according to the O’Driscoll histological cartilage repair scale with Safranin-O stain at weeks 4 and 8. An asterisk (*) denotes a statistically significant difference ( n = 5, * P < 0.05, ** P < 0.01).

Article Snippet: TRIzol reagent and LIVE/DEAD viability/cytotoxicity kit for mammalian cells, mouse COL1 monoclonal antibody (MA1-26771), rabbit type II collagen (COL2) polyclonal antibody (PA1-26206), CD14–phycoerythrin (PE), CD34–PE, CD45–PE, CD73–PE, CD90–PE, CD105–PE, and mouse immunoglobulin G1 kappa isotype control–PE were purchased from Invitrogen (Carlsbad, California, United States).

Techniques: Immunohistochemical staining, Transplantation Assay, Staining, Immunohistochemistry

Journal: Bioengineering

Article Title: Comparison of Bioengineered Scaffolds for the Induction of Osteochondrogenic Differentiation of Human Adipose-Derived Stem Cells

doi: 10.3390/bioengineering11090920

Figure Lengend Snippet: hASC survival and differentiation in GelMA hydrogels. ( A ) CCK8 assay of hASCs encapsulated in the GelMA hydrogel at different time points (1 week intervals). Results are reported as mean ± SEM of n = 3 samples/group. ( B ) Representative confocal imaging of Dead/live fluorescence staining of hASCs in the GelMA hydrogel after 3 weeks of culture. Red cells are dead while green cells are alive. ( C ) Alcian blue staining of GelMA hydrogel after 24 h and 3 weeks of culture. ( D ) Alizarin red staining of GelMA hydrogel after 24 h and 3 weeks of culture. ( E , F ) Confocal imaging of immunofluorescence for COL2A1 (green) and OCN (red) in hASCs encapsulated in GelMA hydrogel after 3 weeks of culture.

Article Snippet: Briefly, samples were incubated with rabbit polyclonal anti-collagen type II alpha 1 (COL2A1) antibody (Merck) and rabbit polyclonal anti-osteocalcin (OCN) antibody (Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: CCK-8 Assay, Imaging, Fluorescence, Staining, Immunofluorescence

Journal: Bioengineering

Article Title: Comparison of Bioengineered Scaffolds for the Induction of Osteochondrogenic Differentiation of Human Adipose-Derived Stem Cells

doi: 10.3390/bioengineering11090920

Figure Lengend Snippet: hASC survival and differentiation in PEGDA scaffold. ( A ) CCK8 assay of hASCs seeded in the PEGDA scaffold at different time points (1 week intervals). Results are reported as mean ± SEM of n = 3 samples/group. T test: * p < 0.05. ( B ) Representative confocal imaging of Dead/live fluorescence staining of hASCs seeded in the PEGDA scaffold after 3 weeks of culture. ( C ) Confocal imaging of immunofluorescence for COL2A1 (green) and OCN (red) in hASCs seeded in the PEGDA scaffold after 3 weeks of culture.

Article Snippet: Briefly, samples were incubated with rabbit polyclonal anti-collagen type II alpha 1 (COL2A1) antibody (Merck) and rabbit polyclonal anti-osteocalcin (OCN) antibody (Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: CCK-8 Assay, Imaging, Fluorescence, Staining, Immunofluorescence

Journal: Bioengineering

Article Title: Comparison of Bioengineered Scaffolds for the Induction of Osteochondrogenic Differentiation of Human Adipose-Derived Stem Cells

doi: 10.3390/bioengineering11090920

Figure Lengend Snippet: hASC survival and differentiation in celery-based scaffold. ( A ) SEM imaging of a single hASC inside a niche of the celery-based scaffold. ( B ) CCK8 assay of hASCs seeded in the celery-based scaffold at different time points (1 week intervals). Results are reported as mean ± SEM of n = 3 samples/group. T test: * p < 0.05. ( C ) Representative confocal imaging of Dead/live fluorescence staining of hASCs seeded in the scaffold after 3 weeks of culture. ( D ) Confocal 3D stack and ( E ) the projection of hASC distribution inside the scaffold. ( F ) Confocal imaging of immunofluorescence for COL2A1 (green) and OCN (red) in hASCs seeded in the PEGDA scaffold after 3 weeks of culture.

Article Snippet: Briefly, samples were incubated with rabbit polyclonal anti-collagen type II alpha 1 (COL2A1) antibody (Merck) and rabbit polyclonal anti-osteocalcin (OCN) antibody (Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: Imaging, CCK-8 Assay, Fluorescence, Staining, Immunofluorescence